Ekstraćelijske vezikule kao biomarkeri aktivnosti bolesti kod vaskulitisa udruženim sa antitelima protiv citoplazme neutrofila

[M. G. Manojlović]

127 list.

References: list.101-123. Biography: list.124.

Pediatrics, Immunology, Rheumatology

The purpose of this scientific investigation was to evaluate the degree of inflammation and disease activity using activity markers on extracellular vesicles in patients with ANCA-positive vasculitis, and to compare findings with healthy controls. The research was conducted in collaboration with Karolinska University Hospital, Solna, Sweden, within ERASMUS plus exchange program between University of Niš and Karolinska Institute. Data and biological samples were obtained from the VASKA cohort, which included patients with active (defined as BVAS≥1, infection excluded) and inactive ANCA vasculitis, i.e. in remission (BVAS=0). Age- and sex-matched healthy individuals served as control subjects. Concentration and phenotype of extracellular vesicles in plasma were assessed by flow cytometry, in both active and inactive disease, and compared to healthy controls. In addition, the procoagulant properties of extracellular vesicles were examined through their effect on thrombin formation, using a modified method based on Calibrated Automated Thrombogram (CAT) assay. The results demonstrated significantly elevated levels of extracellular vesicles bearing PTX3, HMGB1, TWEAK and components of complement system (C3a and C5a) in patients with active disease compared to those in remission and with healthy subjects. This study demonstrates that circulating extracellular vesicles are significantly elevated in active AAV and correlate with clinical disease activity (BVAS). These findings support their potential role as biomarkers for disease monitoring.

extracellular vesicles, ANCA associated vasculitis, biomarkers, disease activity

eкстрацелуларне везикуле, ANCA асоцирани васкулитис, биомаркери, активност болести

612.017:616-053.2(043.3)

B 007; B 660; B 500; B 580

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