Title
Antimikrobna aktivnost komercijalnih etarskih ulja odabranih biljnih vrsta porodice Lamiaceae na kliničke izolate Pseudomonas aeruginosa i Klebsiella spp.: antivirulentni i sinergistički potencijal
Creator
Pejčić Pejić, Milica G., 1991-
CONOR:
121178889
Copyright date
2024
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
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Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 25.10.2024.
Other responsibilities
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Nišu
Faculty
Prirodno-matematički fakultet
Group
Odsek za biologiju i ekologiju
Alternative title
Antimicrobial activity of selected Lamiaceae taxa commercial essential oils against Pseudomonas aeruginosa and Klebsiella spp. clinical isolates> anti-virulence and synergistic potential
Publisher
[Milica G. Pejčić Pejić]
Format
191 list
description
Bibliografija: list. 142-179
description
Microbiology
Abstract (en)
The objectives of this doctoral dissertation were to determine the
chemical composition of commercial basil, sage, and winter savory
essential oils, to establish their antimicrobial and antivirulent potentials
as well as their ability to enhance the activity of the antibiotic
ciprofloxacin in the controlling the growth of Pseudomonas aeruginosa
and Klebsiella spp. clinical isolates. These species represent the most
common pathogenic strains characterized by increased resistance and
are leading causes of chronic infections due to their ability to produce
biofilm.
The chemical analysis of the oils revealed a high prevalence of the
monoterpene class in all three oils and a high content of the components
α-thujone, camphor, linalool, (E)-anethole, thymol and p-cymene.
After their application to P. aeruginosa isolates, basil and sage oils
showed antimicrobial activity in the range of 5-20 mg/ml, while winter
savory oil activity ranged from 0.63 to 10 mg/ml. When applied to
Klebsiella spp. isolates, the activity range of the basil oil was 2.5-10
mg/ml, 1.25-5 mg/ml for winter savory oil, while sage oil was active
only in concentrations higer than 20 mg/ml. The results indicate the
reducing potential of the tested oils in all tested biofilm formation
stages, and that they completely degrade the mature biofilm in P.
aeruginosa isolates, while in Klebsiella spp. that effect is somewhat
lower. The results from the tests investigating the activities of the oils
on other P. aeruginosa virulence factors showed that all three oils
significantly reduce pyocyanin production, while basil and sage oils
showed abilities to reduce motility in P. aeruginosa isolates, which was
not observed after the application of winter savory oil. The tested oils
also led to increased activity of the antibiotic ciprofloxacin after
application to the mentioned clinical isolates.
Authors Key words
Multirezistencija, Pseudomonas aeruginosa, Klebsiella spp.,
faktori virulencije, biofilm, pokreti, piocijanin, etarska
ulja, sinergizam
Authors Key words
Multiresistance, Pseudomonas aeruginosa, Klebsiella spp., virulence
factors, biofilm, motility, pyocyanin, essential oils, synergism
Classification
543.95+665.52:[582.929.4:579.841.1
Subject
B 230
Type
Tekst
Abstract (en)
The objectives of this doctoral dissertation were to determine the
chemical composition of commercial basil, sage, and winter savory
essential oils, to establish their antimicrobial and antivirulent potentials
as well as their ability to enhance the activity of the antibiotic
ciprofloxacin in the controlling the growth of Pseudomonas aeruginosa
and Klebsiella spp. clinical isolates. These species represent the most
common pathogenic strains characterized by increased resistance and
are leading causes of chronic infections due to their ability to produce
biofilm.
The chemical analysis of the oils revealed a high prevalence of the
monoterpene class in all three oils and a high content of the components
α-thujone, camphor, linalool, (E)-anethole, thymol and p-cymene.
After their application to P. aeruginosa isolates, basil and sage oils
showed antimicrobial activity in the range of 5-20 mg/ml, while winter
savory oil activity ranged from 0.63 to 10 mg/ml. When applied to
Klebsiella spp. isolates, the activity range of the basil oil was 2.5-10
mg/ml, 1.25-5 mg/ml for winter savory oil, while sage oil was active
only in concentrations higer than 20 mg/ml. The results indicate the
reducing potential of the tested oils in all tested biofilm formation
stages, and that they completely degrade the mature biofilm in P.
aeruginosa isolates, while in Klebsiella spp. that effect is somewhat
lower. The results from the tests investigating the activities of the oils
on other P. aeruginosa virulence factors showed that all three oils
significantly reduce pyocyanin production, while basil and sage oils
showed abilities to reduce motility in P. aeruginosa isolates, which was
not observed after the application of winter savory oil. The tested oils
also led to increased activity of the antibiotic ciprofloxacin after
application to the mentioned clinical isolates.
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