Title
Modifikacija svojstava stomatoprotetskih akrilata biocidnim agensima na bazi srebra
Creator
Gligorijević, Nikola,
CONOR:
51463177
Copyright date
2023
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 16.10.2024.
Other responsibilities
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za stomatološku protetiku
Alternative title
Modification of denture base resin properites with silver-based biocidal agents: an experimental study
Publisher
[N. M. Gligorijević]
Format
209 str.
description
Biografija autora: str. [209]
Bibliografija: str. 179-205
description
Prosthodontics, dental materials, microbiology, molecular biology
Abstract (en)
The research was based on the assumption that the modification of
commercially available hot and cold polymerizing PMMA materials
by adding silver-based biocidal agents (silver nanoparticles - AgNPs;
silver chloride - AgCl) results in their antimicrobial potential against
microorganisms that cause denture stomatitis, without potentially
toxic effects on oral tissues. The goal of the research was to modify
and test the properties of hot and cold polymerizing PMMA
(examination of the chemical structure, amount and distribution of the
silver-based biocidal agent; surface characteristics of the modified
materials; the effect of the modification on the degree of
polymerization, i.e. the amount of residual monomer; potential release
of silver from the modified materials; biocompatibility testing in in
vitro conditions on cell cultures and testing the antimicrobial potential
of the modified materials against microorganisms isolated from
patients with prosthetic stomatitis as well as commercially available
strains of C. albicans and S. aureus). The material was modified by
incorporating different concentrations of AgNPs and AgCl (2%, 5%
and 10% AgNPs, as well as 10% AgCl).
The analysis of the composition of the oral flora of patients wearing
mobile dental prostheses was performed by taking swabs from the
basal surface of the dental prostheses and the mucous membrane of
the denture support on a sample of 15 patients with clinically
diagnosed prosthetic stomatitis and an analogous control group
without clinical manifestations of this disease. The results of the
analysis of the composition of the oral flora of the denture supporting
tissue in patients with denture stomatitis indicate the presence of C.
albicans on the basal surfaces of mobile dentures as well as on the
surrounding oral tissue. The results of testing the chemical structure
of modified PMMA materials indicate the incorporation of biocidal
agents (AgNPs/AgCl) into the polymer chain with a better distribution
of smaller sized biocidal agents (AgNPs). The color stability of the
modified PMMA indicates a decrease in reflection, i.e. a significant
darkening of the modified materials (AgCl-PMMA CP is 90% darker
than control, while AgNPs-PMMA CP is 130% darker than
unmodified PMMA CP; that is, AgCl-PMMA HP is 100% darker than
control, while AgNPs-PMMA HP is 200% darker than unmodified
PMMA HP), so further work should be done to improve this property.
The results of testing the degree of polymerization of modified
PMMA materials indicate a decrease in the amount of residual
monomer in hot polymerizing PMMA, while modifying cold
polymerizing PMMA resulted in a decrease (10% AgNPs-PMMA)
but also an increase in the amount of residual monomer (10% AgCl-
PMMA). The release of silver from the modified materials did not
occur, so the antimicrobial effect can be explained by direct contact
with the tested microorganisms. The results indicate antimicrobial
activity against commercial strains of C. albicans and S. aureus as
well as C. albicans isolates. Regarding the investigation of the effect
of modified hot and cold polymerizing AgNPs-PMMA and AgCl-
PMMA on the viability and proliferation of L929 cells in cell culture,
the results indicate their unchanged and optimal biocompatibility
compared to the control and unmodified PMMA materials.
The research resulted in chemically and antimicrobial effective
modification of commercially available PMMA with biocidal agents,
without affecting the viability and proliferation of cell culture. This
created a good basis for further testing of modified materials in in vivo
and clinical conditions, which would result in improved acrylate
materials for the production and readaptation of dental prostheses.
Improving the properties of the materials available on the market
would solve the problem of the prevalence of prosthetic stomatitis and
enable its successful prevention and therapy, which would solve a
significant health and socioeconomic challenge.
Authors Key words
PMMA, nanotehnologija, AgNPs, antimikrobna svojstva
Authors Key words
PMMA, nanotechnology, AgNPs, antimicrobial properties
Classification
616.314-76/77:546.57:579(043.3)
Subject
B 730
Type
Tekst
Abstract (en)
The research was based on the assumption that the modification of
commercially available hot and cold polymerizing PMMA materials
by adding silver-based biocidal agents (silver nanoparticles - AgNPs;
silver chloride - AgCl) results in their antimicrobial potential against
microorganisms that cause denture stomatitis, without potentially
toxic effects on oral tissues. The goal of the research was to modify
and test the properties of hot and cold polymerizing PMMA
(examination of the chemical structure, amount and distribution of the
silver-based biocidal agent; surface characteristics of the modified
materials; the effect of the modification on the degree of
polymerization, i.e. the amount of residual monomer; potential release
of silver from the modified materials; biocompatibility testing in in
vitro conditions on cell cultures and testing the antimicrobial potential
of the modified materials against microorganisms isolated from
patients with prosthetic stomatitis as well as commercially available
strains of C. albicans and S. aureus). The material was modified by
incorporating different concentrations of AgNPs and AgCl (2%, 5%
and 10% AgNPs, as well as 10% AgCl).
The analysis of the composition of the oral flora of patients wearing
mobile dental prostheses was performed by taking swabs from the
basal surface of the dental prostheses and the mucous membrane of
the denture support on a sample of 15 patients with clinically
diagnosed prosthetic stomatitis and an analogous control group
without clinical manifestations of this disease. The results of the
analysis of the composition of the oral flora of the denture supporting
tissue in patients with denture stomatitis indicate the presence of C.
albicans on the basal surfaces of mobile dentures as well as on the
surrounding oral tissue. The results of testing the chemical structure
of modified PMMA materials indicate the incorporation of biocidal
agents (AgNPs/AgCl) into the polymer chain with a better distribution
of smaller sized biocidal agents (AgNPs). The color stability of the
modified PMMA indicates a decrease in reflection, i.e. a significant
darkening of the modified materials (AgCl-PMMA CP is 90% darker
than control, while AgNPs-PMMA CP is 130% darker than
unmodified PMMA CP; that is, AgCl-PMMA HP is 100% darker than
control, while AgNPs-PMMA HP is 200% darker than unmodified
PMMA HP), so further work should be done to improve this property.
The results of testing the degree of polymerization of modified
PMMA materials indicate a decrease in the amount of residual
monomer in hot polymerizing PMMA, while modifying cold
polymerizing PMMA resulted in a decrease (10% AgNPs-PMMA)
but also an increase in the amount of residual monomer (10% AgCl-
PMMA). The release of silver from the modified materials did not
occur, so the antimicrobial effect can be explained by direct contact
with the tested microorganisms. The results indicate antimicrobial
activity against commercial strains of C. albicans and S. aureus as
well as C. albicans isolates. Regarding the investigation of the effect
of modified hot and cold polymerizing AgNPs-PMMA and AgCl-
PMMA on the viability and proliferation of L929 cells in cell culture,
the results indicate their unchanged and optimal biocompatibility
compared to the control and unmodified PMMA materials.
The research resulted in chemically and antimicrobial effective
modification of commercially available PMMA with biocidal agents,
without affecting the viability and proliferation of cell culture. This
created a good basis for further testing of modified materials in in vivo
and clinical conditions, which would result in improved acrylate
materials for the production and readaptation of dental prostheses.
Improving the properties of the materials available on the market
would solve the problem of the prevalence of prosthetic stomatitis and
enable its successful prevention and therapy, which would solve a
significant health and socioeconomic challenge.
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