Title
Komparativna analiza genske varijabilnosti i nivoa cirkulišućih faktora nekroze tumora, HSP 70 i Fas/FasL u primarnom glaukomu otvorenog ugla
Creator
Trenkić-Božinović, Marija 1976-
Copyright date
2016
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 21.09.2016.
Other responsibilities
mentor
Zlatanović, Gordana
predsednik komisije
Jovanović, Predrag
član komisije
Jevtović Stoimenov, Tatjana
član komisije
Vukosavljević, Miroslav
član komisije
Jocić Đorđević, Jasmina
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za otorinolaringologiju i oftalmologiju
Alternative title
Comparative analysis of genetic variation and levels of circulating necrosis factor, HSP 70 and Fas/FasL in primary open-angle glaucoma
Publisher
[M. S. Trenkić-Božinović]
Format
123 listа
description
Biografija: list 123
description
Ophthalmology
Abstract (en)
Genetic heterogeneity of primary open-angle glaucoma, and
concentration changes of various inflammation and immune response
mediators in the blood, aqueous humor or tissues, indicate the
involvement of different genetic mechanisms and immune system
activity in the pathogenesis of POAG.
The aim of study was to determine the interdependent clinical,
genetic and biochemical findings in POAG-HTG patients.
Distribution of genetic polymorphisms for TNF-α -308 G/A and -
863 C/A in DNA samples was examined using PCR-RFLP, the
concentrations of circulating TNF-α and HSP 70 in a plasma and
concentration of sFas and sFasL in the aqueous humor were measured
by commercial ELISA tests. Their association with quantitative
clinical parameters was examined.
This study included 357 subjects: 81 POAG-HTG, 35 PEXG, 77
with senile cataract and 164 healthy subjects age-sex matched.
Furthermore, 35 samples of aqueous humor of POAG-HTG patients ,
24 PEXG and 29 with senile cataract were treated.
The results showed that serum concentration of TNF-α was
significantly higher in patients with glaucoma. Genotypes GG TNF-α
(-308) and CC TNF-α (-863) were significantly higher in POAGHTG.
No significant association between TNF-α (-308) G/A and
TNF-α (-863) C/A polymorphism and investigated clinical parameters
was found in POAG-HTG. HSP 70 concentration significantly affects
MD, RNFL Avg, Sup and Inf in POAG-HTG patients. There is a
significant negative correlation between Fas concentration and RNFL
Inf, and negative correlation of FasL with MD and RNFL Avg in
POAG-HTG.
The study concluded that serum TNF-α is a powerful cytokine
with potent significant role in the pathogenesis of glaucoma and
glaucoma neuropathy. HSP 70, sFas and sFasL play a role in
pathogenesis of POAG and may be indicators of the development and
progression of glaucoma neuropathy. It has been shown that TNF-α (-
863) A allelic polymorphism has a protective role in the pathogenesis
of POAG.
Authors Key words
Glaukom otvorenog ugla, faktor nekroze tumora α, genski
polimorfizam, TNF-α-308, TNF-α-863, HSP 70, Fas, FasL
Authors Key words
Open-angle glaucoma, tumor necrosis factor α, genetic
polymorphism, TNF-α-308, TNF-α-863, HSP 70, Fas, FasL
Classification
617.7-007.681:616-006(043.3)
Subject
B 620
Type
elektronska teza
Abstract (en)
Genetic heterogeneity of primary open-angle glaucoma, and
concentration changes of various inflammation and immune response
mediators in the blood, aqueous humor or tissues, indicate the
involvement of different genetic mechanisms and immune system
activity in the pathogenesis of POAG.
The aim of study was to determine the interdependent clinical,
genetic and biochemical findings in POAG-HTG patients.
Distribution of genetic polymorphisms for TNF-α -308 G/A and -
863 C/A in DNA samples was examined using PCR-RFLP, the
concentrations of circulating TNF-α and HSP 70 in a plasma and
concentration of sFas and sFasL in the aqueous humor were measured
by commercial ELISA tests. Their association with quantitative
clinical parameters was examined.
This study included 357 subjects: 81 POAG-HTG, 35 PEXG, 77
with senile cataract and 164 healthy subjects age-sex matched.
Furthermore, 35 samples of aqueous humor of POAG-HTG patients ,
24 PEXG and 29 with senile cataract were treated.
The results showed that serum concentration of TNF-α was
significantly higher in patients with glaucoma. Genotypes GG TNF-α
(-308) and CC TNF-α (-863) were significantly higher in POAGHTG.
No significant association between TNF-α (-308) G/A and
TNF-α (-863) C/A polymorphism and investigated clinical parameters
was found in POAG-HTG. HSP 70 concentration significantly affects
MD, RNFL Avg, Sup and Inf in POAG-HTG patients. There is a
significant negative correlation between Fas concentration and RNFL
Inf, and negative correlation of FasL with MD and RNFL Avg in
POAG-HTG.
The study concluded that serum TNF-α is a powerful cytokine
with potent significant role in the pathogenesis of glaucoma and
glaucoma neuropathy. HSP 70, sFas and sFasL play a role in
pathogenesis of POAG and may be indicators of the development and
progression of glaucoma neuropathy. It has been shown that TNF-α (-
863) A allelic polymorphism has a protective role in the pathogenesis
of POAG.
“Data exchange” service offers individual users metadata transfer in several different formats. Citation formats are offered for transfers in texts as for the transfer into internet pages. Citation formats include permanent links that guarantee access to cited sources. For use are commonly structured metadata schemes : Dublin Core xml and ETUB-MS xml, local adaptation of international ETD-MS scheme intended for use in academic documents.