Comparative analysis of tissue response to collagen-based biomaterials applied in various ways and in different animal models of implantation

[M. B. Radenković Stošić]

[17], 196 listova

Biobibliografija autora: list. 189-193. Bibliografija: list. 161-181.

Biomedicine

Collagen-based biomaterials represents a popular choice for use in tissue engineering, thanks to their biocompatibility, bioresorbability and the possibility of modification during the production process that allows a control of stability and degradation time in the organism. Collagen used for the production of biomaterials can be obtained from various sources, while taking into account the potential application of collagen-based biomaterial, which is conditioned by the method of production and the source from which it was isolated. The aim of this doctoral dissertation was a comparative analysis of the tissue response to collagen-based biomaterials of different species and tissue origin, different production method and structure, as well as with different additions of physiological factors such as blood and blood derivatives. The research was carried out on different experimental animal models, namely orthotopic ones, which involved the implantation of differently prepared collagen-based biomaterials of different origin into the bone defects on rat calvaria and in the rabbit tibia models, as well as the implantation of differently prepared collagen biomaterials of different origin in the subcutaneous implantation models in mice and rats. The tissue response to the implanted collagen-based biomaterials was monitored and analyzed in several different time periods after implantation, and the explanted biomaterial samples with the surrounding tissue were analyzed using histological, immunohistochemical and histomorphometric methods as well as scanning electron microscopy. The results showed that collagen-based biomaterials originating from non-mammalian species, such as jellyfish, can be a suitable alternative source for collagen of mammalian origin in bone and soft tissue regeneration. Collagens of different origins and production methods have different effects on bone tissue repair and causes a different response of subcutaneous and bone tissue, while the addition of blood and blood derivatives affects the resorption time of collagenbased biomaterials and tissue response. These findings emphasize the importance of choosing the appropriate collagen-based biomaterial in accordance with the conditions in which they are applied as well as applied approaches in clinical application in order to achieve the desired results in the field of regenerative medicine.

odgovor tkiva, kolagen, biomaterijali, animalni modeli, defekti kosti, regeneracija kosti, tkivno inženjerstvo, implantati, implantacija

tissue response, collagen, biomaterials, animal models, bone defects, bone regeneration, tissue engineering, implants, implantation

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