Clinical significance of expression of adhesion molecules, Stathmin 1 and neovascularization in colorectal adenocarcinoma

[J. T. Žujović]

201 list

Beleška o autoru: list 198, Bibliografija: listovi 164-197.

Surgery

The aim of our study was to examine the association of the expression of Ki67, E-cadherin, Stathmin1, VEGF and CD105 with clinical-pathological parameters of colorectal adenocarcinoma. In our study was used biopsy operative material from the Center for Abdominal Surgery of the Clinical Center of Montenegro (KCCG) obtained by resection of colorectal cancer. The study group consisted of operative biopsies of colorectal adenocarcinoma (n = 72), and the control group (n = 72) consisted of operative biopsies of non-tumor tissue from the tumor environment. After routine tissue processing and molding into paraffin, the classical HE method and immunohistochemical ABC method with anti-Ki67, Stathmin1, E-cadherin.VEGF and CD105 antibodies were applied on sections 4μm thick tissue. The SPSS software package for Windows was used to analyze the obtained results (ver.19.0 IBMCorp). None of the examined proteins depends on the sex or age of the patient, location of the tumor in the colon, mode of tumor growth or histological grade. Expression of E - cadherina, Stathmin1, Ki67, VEGF and CD105 are significantly associated with Astler-Coller stage (r = 0.875,0.865,0.818,0.872 and 0.630) and with lymph node metastases (r = -0.729.0.725,0.766,0.612 and 0.553). These biomarkers are significantly, but with slightly lower correlation coefficients, associated with pT tumor status, lymphovascular and perineural invasion, and metastases. Stepwise regression analysis showed that E-cadherin, Stathmin1 and Ki67 in combination with with pT status and distant metastases have a the greatest significance for prediction of colorectal cancer progression. ROC analysis showed that the greatest diagnostic performance has Stathmin1 (sensitivity 97.3% and specificity 91.4%), followed by Ki67 and CD105. Our results indicate that, of the examined biomarkers, the strongest prediction significance for progression of colorectal cancer have the expression of E-cadherin, Stathmin1 and Ki67. Based on expression of these proteins it is possible to identify the aggressive phenotype of colorectal cancer, which provides possibility of personalized approach to antitumor treatment, using antagonists of E-cadherina, Stathmin1 and Ki67.

kolorektalni karcinom, ekspresija Ki67, Stathmin1, E-cadherin, VEGF, CD105, prediktori progresije tumora, dijagnostički značaj

colorectal cancer, Ki67 expression, Stathmin1, E-cadherin, VEGF, CD105, predictors of tumor progression, diagnostic significance

616.348/.351-006.6-089:576.3(043.3)

B 600, B 200

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