Title
Ispitivanje interakcije fotoosetljivog nanokompozitnog sistema baziranog na nedopiranim i dopiranim česticama titanijum (IV) oksida s biomolekulima i ćelijama
Creator
Matijević, Milica, 1984-
CONOR:
86767113
Copyright date
2022
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
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Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 30. 05. 2022.
Other responsibilities
predsednik komisije
Nešić, Maja
član komisije
Korićanac, Lela
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Nišu
Faculty
Prirodno-matematički fakultet
Group
Odsek za hemiju
Alternative title
Investigations of the interaction of photo-sensitive nanocomposite system based on
undoped and doped titanium oxide nanoparticles with biomolecules and cells
Publisher
[M. P. Matijević]
Format
126 str.
description
Bibliografija: str. 111-126.
description
General and Inorganic Chemistry
Abstract (en)
Nanostructured inorganic compounds such as TiO2 may be used as drug delivery carriers
and photosensitizers (PSs) in light-based therapy – photodynamic therapy (PDT). This
doctoral dissertation explores the implementation of PDT with unmodified TiO2 (colloidal
nanoparticles, TiO2 NPs; prolate nanospheroids, TiO2 PNSs) and modified (doped
nanoparticles, N- and C-TiO2 NPs; conjugated to a cytostatic model in the nanocomposite
systems, NCSs).
Transmission Electron Microscopy confirmed the nanosize (5-104 nm), whereas X-ray
diffraction and Raman spectroscopy revealed predomination of the most photo-active
form – anatase of the investigated TiO2. Afterwards, NCSs were formed by coordination
of TiO2 NPs and PNS to the Ru(II) complex with cytostatic properties – cisdichlorobis(
2,2'-bipyridyl-4,4'-dicarboxylic acid) ruthenium(II). For the modified TiO2,
UV-Vis spectroscopy and bandgap calculations confirmed the visible light absorption.
Electron paramagnetic resonance spectroscopy revealed the presence of hydroxyl radical
in water suspensions of TiO2 PNSs and C-TiO2 NPs, whereas no reactive oxygen species
were detected in suspensions of TiO2 NPs and N-TiO2 NPs.
For NCS, the long-term constant release profiles of the Ru(II) complex were established
in vitro. Namely, the complex release at pH 7 induced by visible light was slower
compared to UV and light’s absence. Furthermore, additional encapsulation of NCS in the
small unilamellar vesicles significantly suppressed the complex release but with no lightinduced
effect.
Confocal microscopy revealed good cells’ internalization of the assessed TiO2. Viability
tests on non-transformed cells upon the incubation with unmodified TiO2 indicated good
biocompatibility. The photocytotoxic activity of the investigated TiO2 was tested on
several cancer cell lines. The most significant viability reduction has been achieved by the
combined treatment with C-TiO2 NPs and blue light on the HeLa cell line. Moreover, CTiO2
NPs promoted the programmed cells’ death mechanisms, contributing to their
exceptional PS features for PDT.
Authors Key words
Fotodinamička terapija, TiO2, nanokompozitni sistem, Ru(II) kompleks,
energetski procep, reaktivne kiseonične vrste, fotosenziter, citotoksičnost,
vrste ćelijske smrti, ANOVA
Authors Key words
Photodynamic therapy, ТiО2, nanocomposite system, Ru(II) complex, band gap, ROS,
photosensitizer, cytotoxicity, cell death modalities, ANOVA
Classification
(678+661.882):577.1(043.3)
(678+661.882):576.3(043.3)
Subject
P 003, P 360
Type
Tekst
Abstract (en)
Nanostructured inorganic compounds such as TiO2 may be used as drug delivery carriers
and photosensitizers (PSs) in light-based therapy – photodynamic therapy (PDT). This
doctoral dissertation explores the implementation of PDT with unmodified TiO2 (colloidal
nanoparticles, TiO2 NPs; prolate nanospheroids, TiO2 PNSs) and modified (doped
nanoparticles, N- and C-TiO2 NPs; conjugated to a cytostatic model in the nanocomposite
systems, NCSs).
Transmission Electron Microscopy confirmed the nanosize (5-104 nm), whereas X-ray
diffraction and Raman spectroscopy revealed predomination of the most photo-active
form – anatase of the investigated TiO2. Afterwards, NCSs were formed by coordination
of TiO2 NPs and PNS to the Ru(II) complex with cytostatic properties – cisdichlorobis(
2,2'-bipyridyl-4,4'-dicarboxylic acid) ruthenium(II). For the modified TiO2,
UV-Vis spectroscopy and bandgap calculations confirmed the visible light absorption.
Electron paramagnetic resonance spectroscopy revealed the presence of hydroxyl radical
in water suspensions of TiO2 PNSs and C-TiO2 NPs, whereas no reactive oxygen species
were detected in suspensions of TiO2 NPs and N-TiO2 NPs.
For NCS, the long-term constant release profiles of the Ru(II) complex were established
in vitro. Namely, the complex release at pH 7 induced by visible light was slower
compared to UV and light’s absence. Furthermore, additional encapsulation of NCS in the
small unilamellar vesicles significantly suppressed the complex release but with no lightinduced
effect.
Confocal microscopy revealed good cells’ internalization of the assessed TiO2. Viability
tests on non-transformed cells upon the incubation with unmodified TiO2 indicated good
biocompatibility. The photocytotoxic activity of the investigated TiO2 was tested on
several cancer cell lines. The most significant viability reduction has been achieved by the
combined treatment with C-TiO2 NPs and blue light on the HeLa cell line. Moreover, CTiO2
NPs promoted the programmed cells’ death mechanisms, contributing to their
exceptional PS features for PDT.
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