Title
Značaj salivarnih defenzina i katelicidina u proceni rizika za nastanak karijesa kod dece
Creator
Stojković, Branislava 1978-
Copyright date
2020
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 19.01.2021.
Other responsibilities
mentor
Igić, Marija 1970-
predsednik komisije
Tričković Janjić, Olivera
član komisije
Jevtović Stoimenov, Tatjana
član komisije
Ivanović, Mirjana
član komisije
Kostić, Miloš
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za preventivnu i dečju stomatologiju i Ortodonciju
Alternative title
The importance of salivary defensins and cathelicidin for caries risk assessment in children
Publisher
[B. B. Stojković]
Format
119 listova
description
Beleška o autoru: list 119,
Prilog: listovi 114-117,
Bibliografija: listovi 95-112.
description
Preventive and Paediatric Dentistry
Abstract (en)
The study aimed to examine the significance of human α defensin 1 (HNP-1), human β defensin 2 (hBD-2) and human cathelicidin (LL-37) of unstimulated saliva as caries risk biomarkers in childhood.
A one-year, prospective study which included preschool children (N=250) and early adolescents (N=213) was conducted. Having interviewed the mothers/guardians of the subjects, socio-demographic data, data on the health status, oral hygiene, and dietary habits of the subjects were collected. The clinical examination recorded the state of oral hygiene and dental health of the subjects. Unstimulated saliva was sampled to determine its pH value, and in 85 preschool children and 85 early adolescents to determine the salivary level of HNP-1, hBD-2 and LL-37 using commercial ELISA kits. After 12 months, a repeated examination recorded the state of the dental health of the subjects, along with the determination of the annual caries increment.
A cross-sectional study showed that the concentration of the tested HNP-1, hBD-2, LL-37 correlated with the age of the children, and the salivary pH but not with the caries presence, so the study did not determine their significance as caries risk indicators. In preschool age, this significance was shown by gender (p=0.021), the age of the mother (p=0.028), length of breastfeeding (p=0.042), frequency of carbohydrate intake (p=0,009), and values of the oral hygiene index (p=0.042), that is, the level of education of the mother (p<0.001) and the father (p=0.002), oral hygiene index values (p<0.001), frequency (p=0.029) and length of performing oral hygiene (p<0.001) in early adolescence. The logistic regression analysis showed that the analysed peptides were not recognized as reliable caries predictors in childhood. The multivariate model showed that the most significant caries predictors in preschool children were the oral hygiene index (p=0.005) and the absence of dental visits (p=0.014), compared to gender (p=0.001) and variables related to oral hygiene (p=0.044) in early adolescence.
Determining the importance of salivary defensins and cathelicidin as caries risk biomarkers in childhood is complex, since their concentration may be related to certain factors, such as age and salivary pH which the study found, and probably with many salivary components that may interact with these peptides. Therefore, it could be considered justified to use a multimarker approach in determining
the importance of defensins and cathelicidin as caries risk biomarkers in children, rather than analyzing their efficacy as a solely risk biomarkers. Based on the results of the study, socio-demographic characteristics and oral-hygiene habits remain important caries risk factors in childhood, suggesting the importance of the mechanical control of dental biofilm as a central caries preventive measure.
Authors Key words
Karijes, Defenzini, Katelicidin, Pljuvačka, Karijes rizik biomarkeri, Deca
Authors Key words
Caries, Defensins, Cathelicidin, Saliva, Caries risk biomarkers, Children
Classification
616.314-002-053.2:612.313(043.3)
Subject
B 730
Type
Tekst
Abstract (en)
The study aimed to examine the significance of human α defensin 1 (HNP-1), human β defensin 2 (hBD-2) and human cathelicidin (LL-37) of unstimulated saliva as caries risk biomarkers in childhood.
A one-year, prospective study which included preschool children (N=250) and early adolescents (N=213) was conducted. Having interviewed the mothers/guardians of the subjects, socio-demographic data, data on the health status, oral hygiene, and dietary habits of the subjects were collected. The clinical examination recorded the state of oral hygiene and dental health of the subjects. Unstimulated saliva was sampled to determine its pH value, and in 85 preschool children and 85 early adolescents to determine the salivary level of HNP-1, hBD-2 and LL-37 using commercial ELISA kits. After 12 months, a repeated examination recorded the state of the dental health of the subjects, along with the determination of the annual caries increment.
A cross-sectional study showed that the concentration of the tested HNP-1, hBD-2, LL-37 correlated with the age of the children, and the salivary pH but not with the caries presence, so the study did not determine their significance as caries risk indicators. In preschool age, this significance was shown by gender (p=0.021), the age of the mother (p=0.028), length of breastfeeding (p=0.042), frequency of carbohydrate intake (p=0,009), and values of the oral hygiene index (p=0.042), that is, the level of education of the mother (p<0.001) and the father (p=0.002), oral hygiene index values (p<0.001), frequency (p=0.029) and length of performing oral hygiene (p<0.001) in early adolescence. The logistic regression analysis showed that the analysed peptides were not recognized as reliable caries predictors in childhood. The multivariate model showed that the most significant caries predictors in preschool children were the oral hygiene index (p=0.005) and the absence of dental visits (p=0.014), compared to gender (p=0.001) and variables related to oral hygiene (p=0.044) in early adolescence.
Determining the importance of salivary defensins and cathelicidin as caries risk biomarkers in childhood is complex, since their concentration may be related to certain factors, such as age and salivary pH which the study found, and probably with many salivary components that may interact with these peptides. Therefore, it could be considered justified to use a multimarker approach in determining
the importance of defensins and cathelicidin as caries risk biomarkers in children, rather than analyzing their efficacy as a solely risk biomarkers. Based on the results of the study, socio-demographic characteristics and oral-hygiene habits remain important caries risk factors in childhood, suggesting the importance of the mechanical control of dental biofilm as a central caries preventive measure.
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