Title
Uticaj različitih načina pranja okrvavljene tkanine na ekspertizu tragova krvnih mrlja i rezultat DNK analize
Creator
Stojanović, Ivan A. 1981-
Copyright date
2020
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 05.11.2020.
Other responsibilities
mentor
Ilić, Goran 1961-
član komisije
Stojanović, Jovan
član komisije
Zdravković, Miodrag
član komisije
Najman, Stevo
član komisije
Savić, Slobodan
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za sudsku medicinu
Alternative title
The effect of different washing procedures of bloodstained fabric on the expertise of blood trace evidence and result of DNA analysis
Publisher
[I. A. Stojanović]
Format
132 lista
description
Beleška o autoru: list 132;
Bibliografija: listovi 126-131.
description
Forensic medicine
Abstract (en)
In practice, there are often various attempts to remove traces of blood
from items in order to conceal a crime. In the study, samples of
bleached cotton fabric (360 samples in total) were used, which were
then washed through one of six different washing cycles. After a
period of time the samples were analyzed. The macroscopic
appearance of the samples, the reaction of the samples to chemical
tests for the presence of blood, quantity (concentration) of DNA,
DNA degradation index and DNA profiles were analyzed. Machine
washing at a temperature of 95 and 60oC using detergent can remove
visible but not invisible traces of bloodstains up to 10 days old, while
bloodstains aged 30 days cannot be removed in one washing cycle.
Regardless of the method of washing, after washing, the visible
remaining part of the bloodstain will be the dominant circumferential
edge of the area of the original bloodstain. Comparative analysis with
the results of DNA analysis revealed that washing older bloodstains
from the cotton fabric makes it easier to remove the DNA, while the
visual feature of bloodstains originating from hemoglobin is more
difficult to remove in older bloodstains. The statistical significance of
the DNA degradation index of the material remaining in the
bloodstains after washing was revealed at the same time concerning
the washing temperature (95 and 60oC) and the application of the
detergent, with the existence of a synergistic enhancing effect of these
two factors on the DNA degradation index. This pattern is completely
absent in the sample group washed at 30oC. By comparing the results,
it was concluded that different experimental conditions in this study,
namely water temperature and detergent, affect different target parts
of DNA (different STR loci) and thus accelerate the degradation of
DNA. Therefore, early-stage DNA degradation does not occur
uniformly in all parts of the genetic material, but this effect is lost
with the passage of time and degradation becomes more alike in all
parts of the DNA. This research identifies certain new scientific facts
that provide forensic experts with a new perspective on concealing the
traces of crime.
Authors Key words
forenzika, krvna mrlja, analiza obrazaca krvnih mrlja, DNK analiza,
degradacija DNK, deterdžent, mašinsko pranje
Authors Key words
forensic science, bloodstain, bloodstain pattern analysis, DNA
analysis, DNA degradation, detergent, machine washing
Classification
343.983.2(043.3)
Subject
B770
Type
Tekst
Abstract (en)
In practice, there are often various attempts to remove traces of blood
from items in order to conceal a crime. In the study, samples of
bleached cotton fabric (360 samples in total) were used, which were
then washed through one of six different washing cycles. After a
period of time the samples were analyzed. The macroscopic
appearance of the samples, the reaction of the samples to chemical
tests for the presence of blood, quantity (concentration) of DNA,
DNA degradation index and DNA profiles were analyzed. Machine
washing at a temperature of 95 and 60oC using detergent can remove
visible but not invisible traces of bloodstains up to 10 days old, while
bloodstains aged 30 days cannot be removed in one washing cycle.
Regardless of the method of washing, after washing, the visible
remaining part of the bloodstain will be the dominant circumferential
edge of the area of the original bloodstain. Comparative analysis with
the results of DNA analysis revealed that washing older bloodstains
from the cotton fabric makes it easier to remove the DNA, while the
visual feature of bloodstains originating from hemoglobin is more
difficult to remove in older bloodstains. The statistical significance of
the DNA degradation index of the material remaining in the
bloodstains after washing was revealed at the same time concerning
the washing temperature (95 and 60oC) and the application of the
detergent, with the existence of a synergistic enhancing effect of these
two factors on the DNA degradation index. This pattern is completely
absent in the sample group washed at 30oC. By comparing the results,
it was concluded that different experimental conditions in this study,
namely water temperature and detergent, affect different target parts
of DNA (different STR loci) and thus accelerate the degradation of
DNA. Therefore, early-stage DNA degradation does not occur
uniformly in all parts of the genetic material, but this effect is lost
with the passage of time and degradation becomes more alike in all
parts of the DNA. This research identifies certain new scientific facts
that provide forensic experts with a new perspective on concealing the
traces of crime.
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