Title
Ekotoksičnost nanočestica oksida metala i potencijalne metode za njihov biomonitoring u akvatičnim ekosistemima
Creator
Savić-Zdravković, Dimitrija 1990-
Copyright date
2020
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 23.10.2020.
Other responsibilities
mentor
Milošević, Đurađ 1983-
član komisije
Zarubica, Aleksandra 1975-
predsednik komisije
Stojković-Piperac, Milica 1983-
član komisije
Cvetković, Vladimir 1985-
član komisije
Kolarević, Stoimir 1984-
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Nišu
Faculty
Prirodno-matematički fakultet
Group
Odsek za biologiju i ekologiju
Alternative title
Ecotoxicity of metal oxide nanoparticles and potential methods of their biomonitoring in aquatic ecosystems
: doctoral dissertation
Publisher
[D. N. Savić Zdravković]
Format
345 str.
description
Biobibliografija: str. 341-345;
Bibliografija: str. 273-327.
description
Ecotoxicology
Abstract (en)
This research deals with ecotoxicity of insoluble metal oxide nanoparticles, that deposit at the bottom of aquatic ecosystems and therefore influence the hydrobiocenosis community: titanium dioxide nano-TiO2 (used as a food colour), cerium oxide nano-CeO2 (used in the automotive industry) and iron oxide nano-Fe3O4 (magnetic particles manufactured for medicinal use).
Detailed characterisation of examined nanoparticles was conducted through contemporary analytical methods (for the analysis of specific surface-BET; surface chemistry-XPS; phase and crystal structure-XRD; size, aggregation and sample photographing-TEM, SEM and AFM; qualitative and quantitative element analysis-EDX; hydrodynamic diameter, polydispersity index and ζ-potential-DLS; in situ nanoparticle characterisation in the experimental sediment-EDX and SEM). Multiparametric bioassays were conducted, according to the OECD protocol, on aquatic model organisms: Chironomidae (Diptera): Chironomus tentans and Chironomus riparius. A series of standard (mortality, survival rate, developmental time and rate and emergence delay), as well as nonstandard biomarkers were analysed (oxidative stress-lipid and protein oxidation, SOD and CAT activity, expressed by total protein content; in vivo genotoxicity via DNA damage analysis; haemoglobin concentration; morphological variability change). For the first time in known literature geometric morphometric method was used for comprehensive nanoparticle toxicity analysis.
Bioaccumulation of all tested nanoparticles by model organisms has been proven. The sublethal toxicity of titanium, cerium and iron oxide nanoparticles was determined, while in the case of nano-TiO2, a lethal effect was observed as well. Modified OECD protocol number 218 that implies using C. riparius as model organism, fine quartz sand substrate, detailed nanoparticle characterization, bioaccumulation analysis, analysis of standard and complementary sublethal biomarkers, enables efficient assessment of toxicity and monitoring of nanoparticles in aquatic ecosystems.
Authors Key words
Nanočestice, hironomide, Chironomus, biološki testovi, biomarkeri, OECD protokol, geometrijska morfometrija, titanijum dioksid TiO2, cerijum oksid CeO2, gvožđe oksid Fe3O4
Authors Key words
nanoparticles, aquatic midges, Chironomus, bioassays, biomarkers, OECD protocol, geometric morphometrics, titanium dioxide TiO2, cerium oxide CeO2, iron oxide Fe3O4
Classification
502/504:615.9+546.6:574.57
Subject
B 003; B 260
Type
Tekst
Abstract (en)
This research deals with ecotoxicity of insoluble metal oxide nanoparticles, that deposit at the bottom of aquatic ecosystems and therefore influence the hydrobiocenosis community: titanium dioxide nano-TiO2 (used as a food colour), cerium oxide nano-CeO2 (used in the automotive industry) and iron oxide nano-Fe3O4 (magnetic particles manufactured for medicinal use).
Detailed characterisation of examined nanoparticles was conducted through contemporary analytical methods (for the analysis of specific surface-BET; surface chemistry-XPS; phase and crystal structure-XRD; size, aggregation and sample photographing-TEM, SEM and AFM; qualitative and quantitative element analysis-EDX; hydrodynamic diameter, polydispersity index and ζ-potential-DLS; in situ nanoparticle characterisation in the experimental sediment-EDX and SEM). Multiparametric bioassays were conducted, according to the OECD protocol, on aquatic model organisms: Chironomidae (Diptera): Chironomus tentans and Chironomus riparius. A series of standard (mortality, survival rate, developmental time and rate and emergence delay), as well as nonstandard biomarkers were analysed (oxidative stress-lipid and protein oxidation, SOD and CAT activity, expressed by total protein content; in vivo genotoxicity via DNA damage analysis; haemoglobin concentration; morphological variability change). For the first time in known literature geometric morphometric method was used for comprehensive nanoparticle toxicity analysis.
Bioaccumulation of all tested nanoparticles by model organisms has been proven. The sublethal toxicity of titanium, cerium and iron oxide nanoparticles was determined, while in the case of nano-TiO2, a lethal effect was observed as well. Modified OECD protocol number 218 that implies using C. riparius as model organism, fine quartz sand substrate, detailed nanoparticle characterization, bioaccumulation analysis, analysis of standard and complementary sublethal biomarkers, enables efficient assessment of toxicity and monitoring of nanoparticles in aquatic ecosystems.
“Data exchange” service offers individual users metadata transfer in several different formats. Citation formats are offered for transfers in texts as for the transfer into internet pages. Citation formats include permanent links that guarantee access to cited sources. For use are commonly structured metadata schemes : Dublin Core xml and ETUB-MS xml, local adaptation of international ETD-MS scheme intended for use in academic documents.