Title
Molekularna i morfološka karakterizacija evropskih vrsta roda Binodoxys Mackauer (Hymenoptera: Bracondiae: Aphidiinae)
Creator
Lazarević, Maja 1991-
Copyright date
2019
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 07.02.2020.
Other responsibilities
mentor
Tomanović, Željko 1966-
član komisije
Ilić-Milošević, Marijana 1983-
član komisije
Stanković, Saša 1982-
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Nišu
Faculty
Prirodno-matematički fakultet
Group
Odsek za biologiju i ekologiju
Alternative title
Molecular and morphological characterization of european species of the genus Binodoxys Mackauer (Hymenoptera: Bracondiae: Aphidiinae)
Publisher
[M. J. Lazarević]
Format
[14], 101, [3] lista
description
Biografija autora: list 101;
Bibliografija: listovi [1-3].
description
Zoology
Abstract (en)
The genera Binodoxys assembles 70 species of Holarctic distribution. Based on the presence of secondary tubercles on the second metasomal segment (petiole), genera Binodoxys can be discriminated from the Trioxys species. The position of the primary and secondary tubercles is taxonomic character for the separation of Binodoxys species. Based on the morphology of the petiole and other morphological characters, eight species have been described in European fauna. The main objective of this study is to determent the molecular and morphological differences between the six European species. Morphologically, using the geometric morphomterics method, 111 petioles and 175 forewings of females were analysed. Molecular analysis was applied using the barcoding COI gene. The geometric morphometrics method of petioles showed the existence of two morpho-groups: „angelicae“ (B. angelicae и B. centaureae) and „acalephae“ (B. acalephae, B. brevicornis, B. heraclei и B. similis). Species from the „angelicae“ morpho-group have elongated petioles long distance between the primary and secondary tubercles, while the petioles of “acalephae” morpho-group are short and with smaller intertubercular distance. The geometric morphometrics of forewings, showed that the B. similis has similar wing morphology to that in “angelicae” morpho-group. Forewings of “angelicae” morpho-group were narrowed, and with longer radial sector vein, when compared to the members of “acalephae” group. Molecular analysis confirmed the existence of six species, which are discriminated in two phylogenetic clades. The first clade clusters two species (B. angelicae and B. centaureae), while the remaining four species are grouped in the second lineage (B. acalephae + (B. heraclei + (B. brevicornis + B. similis))). Mapping the phylogeny over the morphological data showed that phylogenetically closer species have a similar petiole shape, but not forewings. Scanning electron microscopy revealed differences in shape and size of the heads and ovipositor. Members of “angelicae” phylo-morpho-group have long petioles and heads, but short ovipositors. Based on all the analyses applied, the existence of six Binodxys species classified into two phylo-morpho groups was confirmed.
Authors Key words
Binodoxys, геометријска морофметрија, петиолуси, крила, COI ген, SEM
Authors Key words
Binodoxys, the geometric morphometrics, petioles, wings, COI, SEM
Classification
595.79:[591.557:595.7(4)(043.3)
Subject
B005; B250; B320
Type
Tekst
Abstract (en)
The genera Binodoxys assembles 70 species of Holarctic distribution. Based on the presence of secondary tubercles on the second metasomal segment (petiole), genera Binodoxys can be discriminated from the Trioxys species. The position of the primary and secondary tubercles is taxonomic character for the separation of Binodoxys species. Based on the morphology of the petiole and other morphological characters, eight species have been described in European fauna. The main objective of this study is to determent the molecular and morphological differences between the six European species. Morphologically, using the geometric morphomterics method, 111 petioles and 175 forewings of females were analysed. Molecular analysis was applied using the barcoding COI gene. The geometric morphometrics method of petioles showed the existence of two morpho-groups: „angelicae“ (B. angelicae и B. centaureae) and „acalephae“ (B. acalephae, B. brevicornis, B. heraclei и B. similis). Species from the „angelicae“ morpho-group have elongated petioles long distance between the primary and secondary tubercles, while the petioles of “acalephae” morpho-group are short and with smaller intertubercular distance. The geometric morphometrics of forewings, showed that the B. similis has similar wing morphology to that in “angelicae” morpho-group. Forewings of “angelicae” morpho-group were narrowed, and with longer radial sector vein, when compared to the members of “acalephae” group. Molecular analysis confirmed the existence of six species, which are discriminated in two phylogenetic clades. The first clade clusters two species (B. angelicae and B. centaureae), while the remaining four species are grouped in the second lineage (B. acalephae + (B. heraclei + (B. brevicornis + B. similis))). Mapping the phylogeny over the morphological data showed that phylogenetically closer species have a similar petiole shape, but not forewings. Scanning electron microscopy revealed differences in shape and size of the heads and ovipositor. Members of “angelicae” phylo-morpho-group have long petioles and heads, but short ovipositors. Based on all the analyses applied, the existence of six Binodxys species classified into two phylo-morpho groups was confirmed.
“Data exchange” service offers individual users metadata transfer in several different formats. Citation formats are offered for transfers in texts as for the transfer into internet pages. Citation formats include permanent links that guarantee access to cited sources. For use are commonly structured metadata schemes : Dublin Core xml and ETUB-MS xml, local adaptation of international ETD-MS scheme intended for use in academic documents.