Title
Efekat melatonina na parametre oksidativnog oštećenja, inflamacije i neoangiogeneze u retini pacova sa dijabetesom melitusom tip 2 izazvanim streptozotocin-nikotinamidom
Creator
Đorđević, Branka S.
Copyright date
2019
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 19.11.2019.
Other responsibilities
mentor
Sokolović, Dušan
predsednik komisije
Cvetković, Tatjana
član komisije
Jevtović Stoimenov, Tatjana
član komisije
Kocić, Radivoj
član komisije
Kostić, Danijela
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za biohemiju
Alternative title
The effect of melatonin on parameters of oxidative damage, inflammation and neoangiogenesis in the retina of rats with streptozotocin/nicotinamide induced type 2 diabetes mellitus
Publisher
[B. S. Đorđević]
Format
155 listova
description
Beleška o autoru: list 154;
Bibliografija: listovi 113-153.
description
Biochemistry
Abstract (en)
Diabetic retinopathy is the commonest cause of blindness in the
population aged 20 to 79 years. The molecular basis of the disease
is metabolic dysfunction caused by hyperglycemia, which leads to
oxidative damage of biomolecules and inflammation in the retina.
Oxidative stress and inflammation cause damage to the bloodretinal
barrier, impaired blood flow and ischemia in the retina, while
vascular endothelial growth factor (VEGF) is considered to be the
most important factor of disease progression. Melatonin is a
hormone that is synthesized in the pineal gland and retina. It is
considered a circadian rhythm regulator, but it also exerts
significant antioxidant and anti-inflammatory effects and is easily
transported through the blood-retinal barrier due to its physiochemical
properties. The aim of this study is to determine the effects
of melatonin supplementation on the parameters of serum glucose
and lipid metabolism, as well as on the parameters of oxidative
damage, inflammation, and neoangiogenesis in retina of rats with
streptozotocin-nicotinamide induced type 2 diabetes mellitus.
Besides from hyperglycemia and dyslipidemia, a reduced serum
melatonin concentration was found in experimental animals with
type 2 diabetes mellitus. Oral supplementation with melaton at a
dose of 0.2 mg/kg of body weight led to a significant increase in
serum melatonin concentration. Animals treated with melatonin had
reduced serum tumor necrosis factor α (TNF-α) levels, which is
important due to the impact of this proinflammatory cytokine on the
blood-retinal barrier permeability and the leukostasis. Melatonin
reduced the concentration of oxidative damage markers, in particular the lipid peroxidation (thiobarbituric acid reactive substances, TBARS) and protein oxidation (advanced oxidation
protein products, AOPP) markers in the retina, ,as well as, the
concentration of an enzyme inducible nitric oxide synthase (iNOS ),
which contributes to the oxidative damage through the production
of reactive nitrogen species. Melatonin, in addition, leads to a
decrease in the retinal concentration of VEGF and the matrix of
metalloproteinase 9 (MMP-9), which prevents the damage to the
blood-retinal barrier and the neoangiogenesis. The use of melatonin
can, therefore, be considered in the prevention or in the therapy of
changes in the retina associated with type 2 diabetes mellitus.
Authors Key words
melatonin, dijabetesna retinopatija, dijabetes melitus tip 2, retina,
oksidativni stres, VEGF, MMP-9, sICAM-1, iNOS, TNF-α
Authors Key words
melatonin, diabetic retinopathy, type 2 diabetes mellitus, retina,
oxidative stress, VEGF, MMP-9, sICAM-1, iNOS, TNF-α
Classification
615.35:[617.735:616.379-008.64(043.3)
Subject
B480, B620, B740, P004
Type
Tekst
Abstract (en)
Diabetic retinopathy is the commonest cause of blindness in the
population aged 20 to 79 years. The molecular basis of the disease
is metabolic dysfunction caused by hyperglycemia, which leads to
oxidative damage of biomolecules and inflammation in the retina.
Oxidative stress and inflammation cause damage to the bloodretinal
barrier, impaired blood flow and ischemia in the retina, while
vascular endothelial growth factor (VEGF) is considered to be the
most important factor of disease progression. Melatonin is a
hormone that is synthesized in the pineal gland and retina. It is
considered a circadian rhythm regulator, but it also exerts
significant antioxidant and anti-inflammatory effects and is easily
transported through the blood-retinal barrier due to its physiochemical
properties. The aim of this study is to determine the effects
of melatonin supplementation on the parameters of serum glucose
and lipid metabolism, as well as on the parameters of oxidative
damage, inflammation, and neoangiogenesis in retina of rats with
streptozotocin-nicotinamide induced type 2 diabetes mellitus.
Besides from hyperglycemia and dyslipidemia, a reduced serum
melatonin concentration was found in experimental animals with
type 2 diabetes mellitus. Oral supplementation with melaton at a
dose of 0.2 mg/kg of body weight led to a significant increase in
serum melatonin concentration. Animals treated with melatonin had
reduced serum tumor necrosis factor α (TNF-α) levels, which is
important due to the impact of this proinflammatory cytokine on the
blood-retinal barrier permeability and the leukostasis. Melatonin
reduced the concentration of oxidative damage markers, in particular the lipid peroxidation (thiobarbituric acid reactive substances, TBARS) and protein oxidation (advanced oxidation
protein products, AOPP) markers in the retina, ,as well as, the
concentration of an enzyme inducible nitric oxide synthase (iNOS ),
which contributes to the oxidative damage through the production
of reactive nitrogen species. Melatonin, in addition, leads to a
decrease in the retinal concentration of VEGF and the matrix of
metalloproteinase 9 (MMP-9), which prevents the damage to the
blood-retinal barrier and the neoangiogenesis. The use of melatonin
can, therefore, be considered in the prevention or in the therapy of
changes in the retina associated with type 2 diabetes mellitus.
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