Title
Razvojne karakteristike krvnih i limfnih sudova jetre embriona i fetusa čoveka - imunohistohemijska i morfpmetrijsko istraživanje
Creator
Jović, Marko D. 1982-
Copyright date
2018
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 17.09.2018.
Other responsibilities
mentor
Nikolić, Ivan
član komisije
Mojsilović, Marijola
član komisije
Gligorijević, Jasmina
član komisije
Bajčetić, Miloš
član komisije
Petrović, Aleksandar
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za histologiju sa embriologijom
Alternative title
Development characteristics of blood and lymph vessels of embryonal and fetal human liver - an immunohistochemical and morphometric research
Publisher
[М. D. Јović]
Format
201 str.
description
Biografija: str. 201;
Bibliografija: str. 177-200.
description
Histology and embryology
Abstract (en)
In the scientific literature, there are few, and often contradictory data of the development of blood and lymphatic vessels of the human liver. The goal was to define the appearance of different types of blood and lymph vessels and to describe the structure of their walls, in the prenatal development of the liver. The material consisted of livers of 5 embryos and 25 fetuses of different gestational age, sorted by trimesters. In addition to the classical HE method, tissue sections were stained with immunohistochemical methods for the identification of endothelial cells (CD31, CD34, D2-40, LYVE-1), smooth muscle cells (α-smooth muscle actin), pericytes (CD146) and extracellular matrix components (collagen I, III, IV, and laminin) of blood and lymph vessels and synaptophysin for nerves. By the morphometric analyses, it was determined the values of volume and numerical areal density of blood and lymph vessels of the liver. The results of the study show qualitative and quantitative changes regarding the representation of different types of cells and components of the extracellular matrix of the walls of the blood and lymph vessels in the liver during prenatal development. Different phenotypes of endothelial cells of sinusoids have been demonstrated in relation to endothelial of portal and collecting blood venous and lymph vessels, determined on the basis of differences in CD34 and LYVE-1 immunoreactivity. Special attention is dedicated to the distinction between periportal sinusoids and terminal portal venules, which is a novelty in the interpretation of the developmental characteristics of the blood vessels of the human liver.
Authors Key words
Jetra, krvni sudovi, humani embrion i fetus, imunohistohemija
Authors Key words
Liver, blood vessels, human embryo and fetus, immunohistochemistry
Classification
611.018:[611.1::611.36-053.13(043.3)
Subject
В 007, В 210, В 440
Type
Tekst
Abstract (en)
In the scientific literature, there are few, and often contradictory data of the development of blood and lymphatic vessels of the human liver. The goal was to define the appearance of different types of blood and lymph vessels and to describe the structure of their walls, in the prenatal development of the liver. The material consisted of livers of 5 embryos and 25 fetuses of different gestational age, sorted by trimesters. In addition to the classical HE method, tissue sections were stained with immunohistochemical methods for the identification of endothelial cells (CD31, CD34, D2-40, LYVE-1), smooth muscle cells (α-smooth muscle actin), pericytes (CD146) and extracellular matrix components (collagen I, III, IV, and laminin) of blood and lymph vessels and synaptophysin for nerves. By the morphometric analyses, it was determined the values of volume and numerical areal density of blood and lymph vessels of the liver. The results of the study show qualitative and quantitative changes regarding the representation of different types of cells and components of the extracellular matrix of the walls of the blood and lymph vessels in the liver during prenatal development. Different phenotypes of endothelial cells of sinusoids have been demonstrated in relation to endothelial of portal and collecting blood venous and lymph vessels, determined on the basis of differences in CD34 and LYVE-1 immunoreactivity. Special attention is dedicated to the distinction between periportal sinusoids and terminal portal venules, which is a novelty in the interpretation of the developmental characteristics of the blood vessels of the human liver.
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