Title
Biološka aktivnost depsidona i atranorina izolovanih iz lišaja Hypogymnia physodes
Creator
Stojanović, Igor Ž. 1985-
Copyright date
2017
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 09.06.2017.
Other responsibilities
mentor
Šmelcerović, Andrija
predsednik komisije
Agbaba, Danica
član komisije
Najman, Stevo
član komisije
Kocić, Gordana
član komisije
Arsić, Ivana
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za farmaciju
Alternative title
Biological activity of depsidones and atranorin isolated from lichen Hypogymnia physodes
Publisher
[I. Stojanović]
Format
[10], 89, [4] listova
description
Biografija [autora]: listovi [90-93]
description
Pharmaceutical chemistry
Abstract (en)
The objectives of the doctoral dissertation were achieved by determining the chemical composition of the extracts of H. physodes, isolation, structural determination and testing of the biological activity of physodalic acid, physodic acid, 3-hydroxyphysodic acid and atranorin. In this dissertation antioxidant activity, cytotoxic activity on cells of human cervix carcinoma and on rat thymocytes, anticholinesterase activity, antimicrobial activity and influence on the formation of micronuclei in lymphocytes of human blood plasma of physodalic acid, physodic acid, 3-hydroxyphysodic acid and atranorin were investigated The antioxidant activity and anticholinesterase activity were investigated by spectrophotometric methods. Cytotoxic activity on cells of human cervix carcinoma and rat thymocytes was assayed by a colorimetric MTT assay, and CCK-8 assay, respectively. The impact on the formation of micronuclei in lymphocytes of human blood plasma was investigated by micronucleus test. Antimicrobial activity was tested by using microdilution method. Comparing the literature available to us, and these results, it was found that for the first time was investigating the effect of Hypogymnia physodes methanol extract on cells of human cervical cancer and the number of micronuclei in lymphocytes of human blood plasma, impact of atranorin on the number of micronuclei in lymphocytes of human blood plasma and serum activity cholinesterase, antioxidant activity of 3-hydroxyphysodic acid and its effects on rat thymocytes, the number of micronuclei in lymphocytes of human blood plasma, the cells of the human cervical cancer and the activity of serum cholinesterase and the impact physodic acid on rat thymocytes, the number of micronuclei in human lymphocytes blood plasma, the cells of the human cervical cancer and serum cholinesterase activity and antioxidant activity of physodalic acid and its effect on rat thymocytes, the number of micronuclei in lymphocytes of human blood plasma, the cells of the human cervical cancer and the activity of serum cholinesterase.
3-Hydroxyphysodic acid shows a greater anti-oxidant activity from a commercial antioxidant 3,5-di-tert-butyl-4-hydroxytoluene (BHT), and reduce the number of micronucleus cells in human blood plasma
2.2 times more than the commercial protectant amifostine, recommending it as good candidate for in vivo experiments.
Authors Key words
Hypogymnia physodes; fizodalična kiselina; fizodična kiselina; 3-hidroksifizodična kiselina; atranorin; antioksidantna aktivnost; citotoksična aktivnost; antiholinesterazna aktivnost; mikronukleusni test; antimikrobna aktivnost
Authors Key words
Hypogymnia physodes; physodalic acid; physodic acid; 3-hydroxyphysodic acid; atranorin; antioxidant activity; citotoxic activity; anticholinesterase activity; micronucleus test; antimicrobial activity
Classification
577.12:582.282.13(043.3)
Subject
B 740
Type
Elektronska teza
Abstract (en)
The objectives of the doctoral dissertation were achieved by determining the chemical composition of the extracts of H. physodes, isolation, structural determination and testing of the biological activity of physodalic acid, physodic acid, 3-hydroxyphysodic acid and atranorin. In this dissertation antioxidant activity, cytotoxic activity on cells of human cervix carcinoma and on rat thymocytes, anticholinesterase activity, antimicrobial activity and influence on the formation of micronuclei in lymphocytes of human blood plasma of physodalic acid, physodic acid, 3-hydroxyphysodic acid and atranorin were investigated The antioxidant activity and anticholinesterase activity were investigated by spectrophotometric methods. Cytotoxic activity on cells of human cervix carcinoma and rat thymocytes was assayed by a colorimetric MTT assay, and CCK-8 assay, respectively. The impact on the formation of micronuclei in lymphocytes of human blood plasma was investigated by micronucleus test. Antimicrobial activity was tested by using microdilution method. Comparing the literature available to us, and these results, it was found that for the first time was investigating the effect of Hypogymnia physodes methanol extract on cells of human cervical cancer and the number of micronuclei in lymphocytes of human blood plasma, impact of atranorin on the number of micronuclei in lymphocytes of human blood plasma and serum activity cholinesterase, antioxidant activity of 3-hydroxyphysodic acid and its effects on rat thymocytes, the number of micronuclei in lymphocytes of human blood plasma, the cells of the human cervical cancer and the activity of serum cholinesterase and the impact physodic acid on rat thymocytes, the number of micronuclei in human lymphocytes blood plasma, the cells of the human cervical cancer and serum cholinesterase activity and antioxidant activity of physodalic acid and its effect on rat thymocytes, the number of micronuclei in lymphocytes of human blood plasma, the cells of the human cervical cancer and the activity of serum cholinesterase.
3-Hydroxyphysodic acid shows a greater anti-oxidant activity from a commercial antioxidant 3,5-di-tert-butyl-4-hydroxytoluene (BHT), and reduce the number of micronucleus cells in human blood plasma
2.2 times more than the commercial protectant amifostine, recommending it as good candidate for in vivo experiments.
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