Title
Hemijski sastav, antioksidativna, antimikrobna i antiholinesterazna aktivnost biljnih vrsta Seseli rigidum i Seseli pallasii
Creator
Ilić, Marija D. 1983-
Copyright date
2016
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 16.03.2017.
Other responsibilities
mentor
Stankov-Jovanović, Vesna 1968-
član komisije
Mitić, Violeta 1968-
član komisije
Stojanović, Gordana 1960-
član komisije
Jovanović, Olga 1954-
član komisije
Nikolić-Mandić, Snežana
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Nišu
Faculty
Prirodno-matematički fakultet
Group
Odsek za hemiju
Alternative title
Chemical composition, antioxidant, antimicrobial and anticholinesterase activity of plant species Seseli rigidum and Seseli pallasii
Publisher
[M. D. Ilić]
Format
196 str.
description
Biografija sa bibliografijom: str. 187-194
description
Analytical chemistry
Abstract (en)
Тhe chemical composition of essential oils, extracts of different
polarity, and mineral composition of plants Seseli rigidum and Seseli
pallasii were analysed. For rapid analysis of volatiles of S. rigidum
above ground parts, and S. pallasii fruits and roots volatiles, HS/GCMS
technique may be used, while in all other cases is necessary to
isolate essential oils and apply GC-MS technique. According to
principle component analysis (PCA) and hierarchical cluster analysis
(HCA) a groupings of the samples were done, based on the similarity
of the chemical composition of certain plant parts (leaf, flower, fruit
and roots). The variables based on the dominant components were
grouped using PCA and HCA analysis. Biological activities and
characteristics of essential oils, extracts of different polarity of
vegetative parts of both plants were investigated. Antioxidant
properties were tested using ABTS, DPPH, total reducing power
methods, total flavonoids and total phenols content. According to the
ABTS and DPPH methods, the best properties were manifested by
hexane extracts of both tested plant species, and flower acetone
extract of S. rigidum, respectively, while the highest total reducing
capacity had the methanol extracts of S. rigidum. The highest total
phenols and flavonoids were registered for hexane extracts of root and
leaf of S. pallasii. Both applied methods of statistical analyses (PCA
and HCA) in which the results of antioxidant activities were used as
variables, clearly distinguished DPPH method from other methods,
and tested samples were separated by type (oils and extracts), and
according to the type of solvent used in the preparation of extracts.
Determination of antimicrobial activity of essential oils and extracts
versus different strains of Gram (+) and Gram (-) bacteria, using the
microdilution method, showed that the root essential oil of S. rigidum
had the most pronounced effect. In terms of inhibition of enzyme
cholinesterase, estimated using Elman’s method, the highest degree of
inhibition was recorded for the root methanol extract of S. pallasii.
Overall, the best characteristics among tested activities, showed
secondary metabolites of S. rigidum root, and therefore identification
of main components of the extract using liquid chromatography
combined with high resolution mass spectrometry was performed.
Reliably was established presence of thirty compounds, most of
which with coumarin structure. In each of the vegetative parts of both
plants content of macro-, microelements and heavy metals were
determined and found that their content does not exceed the
permissible values. Results obtained using two methods of
multivariate statistics are in accordance and grouped certain plants’
parts based on the highest content of micro, macroelements or heavy
metals.
Authors Key words
Seseli rigidum, Seseli pallasii, etarska ulja, ekstrakti, gasna
hromatografija, tečna hromatografija, masena spektrometrija,
spektrometrija kuplovane plazme, antioksidativna aktivnost,
antimikrobna aktivnost, antiholinesterazna aktivnost,
mikroelementi, makroelementi, teški metali, analiza glavne
komponente, klaster analiza
Authors Key words
Seseli rigidum, Seseli pallasii, essential oils, extracts, gas
chromatography, liquid chromatography, mass spectrometry,
inductively coupled plasma spectrometry, antioxidant activity,
antimicrobial activity, anticholinesterase activity, microelements,
macroelemets, heavy metals, principle component analysis, cluster
analysis
Type
Elektronska teza
Abstract (en)
Тhe chemical composition of essential oils, extracts of different
polarity, and mineral composition of plants Seseli rigidum and Seseli
pallasii were analysed. For rapid analysis of volatiles of S. rigidum
above ground parts, and S. pallasii fruits and roots volatiles, HS/GCMS
technique may be used, while in all other cases is necessary to
isolate essential oils and apply GC-MS technique. According to
principle component analysis (PCA) and hierarchical cluster analysis
(HCA) a groupings of the samples were done, based on the similarity
of the chemical composition of certain plant parts (leaf, flower, fruit
and roots). The variables based on the dominant components were
grouped using PCA and HCA analysis. Biological activities and
characteristics of essential oils, extracts of different polarity of
vegetative parts of both plants were investigated. Antioxidant
properties were tested using ABTS, DPPH, total reducing power
methods, total flavonoids and total phenols content. According to the
ABTS and DPPH methods, the best properties were manifested by
hexane extracts of both tested plant species, and flower acetone
extract of S. rigidum, respectively, while the highest total reducing
capacity had the methanol extracts of S. rigidum. The highest total
phenols and flavonoids were registered for hexane extracts of root and
leaf of S. pallasii. Both applied methods of statistical analyses (PCA
and HCA) in which the results of antioxidant activities were used as
variables, clearly distinguished DPPH method from other methods,
and tested samples were separated by type (oils and extracts), and
according to the type of solvent used in the preparation of extracts.
Determination of antimicrobial activity of essential oils and extracts
versus different strains of Gram (+) and Gram (-) bacteria, using the
microdilution method, showed that the root essential oil of S. rigidum
had the most pronounced effect. In terms of inhibition of enzyme
cholinesterase, estimated using Elman’s method, the highest degree of
inhibition was recorded for the root methanol extract of S. pallasii.
Overall, the best characteristics among tested activities, showed
secondary metabolites of S. rigidum root, and therefore identification
of main components of the extract using liquid chromatography
combined with high resolution mass spectrometry was performed.
Reliably was established presence of thirty compounds, most of
which with coumarin structure. In each of the vegetative parts of both
plants content of macro-, microelements and heavy metals were
determined and found that their content does not exceed the
permissible values. Results obtained using two methods of
multivariate statistics are in accordance and grouped certain plants’
parts based on the highest content of micro, macroelements or heavy
metals.
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