Title
Prednosti upotrebe biotehnološkog postupka produkcije interferona-gama iz prečišćene suspenzije humanih leukocita
Creator
Stanković, Bratislav P. 1959-
Copyright date
2015
Object Links
Select license
Autorstvo 3.0 Srbija (CC BY 3.0)
License description
Dozvoljavate umnožavanje, distribuciju i javno saopštavanje dela, i prerade, ako se navede ime autora na način odredjen od strane autora ili davaoca licence, čak i u komercijalne svrhe. Ovo je najslobodnija od svih licenci. Osnovni opis Licence: http://creativecommons.org/licenses/by/3.0/rs/deed.sr_LATN Sadržaj ugovora u celini: http://creativecommons.org/licenses/by/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 11.12.2015.
Other responsibilities
mentor
Kamenov, Borislav
član komisije
Marjanović, Goran
član komisije
Kocić, Gordana
član komisije
Stanković-Đorđević, Dobrila
član komisije
Romić, Marija
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Alternative title
The advantage of using biotechnological treatment of interferon-gamma from a refined suspension of human leukocytes
Publisher
[B. P. Stanković]
Format
126 listova
description
Beleška o autoru: listovi 125-126
description
Transfusiology, Immunology
Abstract (en)
Interferons (IFN) are first described in the late fifties of the twentieth century as special proteins in the family of cytokines and induced by the host, after invasion virus. If you are transferred to the fresh tissue was observed antiviral activity, thanks to inhibit replication of the virus. In this appearance immunology known as "interference". Under normal conditions in the human body concentration of IFN-a was below detectable levels or because cells synthesize IFN or contain as its constituent element. IFN was divided in three classes: IFN-, IFN- and IFN-. Most mammalian cells formed in unequal quantities of IFN- and IFN- after stimulation with viruses or other agents, or inducers of the synthesis of IFN.
In many countries, is still detained biotechnological process for the production of human IFN (hlFN) for the clinical application of human leukocytes allocated from canned whole blood units, not older than 6 hours. After centrifugation the procedure centrifugation devotes a buffy coat (leukocyte suspension) as a "side" -produkat. Further processing of "buffy coat" (BC) was purified from the red cell contamination, in order to obtain as pure substrate of human leukocytes, which is subjected to viral infection in order to stimulate the synthesis of hIFN-. Different viruses are used as inductors synthesis hIFN-, but in practice most often used "Sendai" virus, cultivated in horion-alantoic membrane of chicken embryos. Induction of synthesis hIFN- played on temperature of +37 ° C and lasts 18 hours. Refined suspension of human tal and practical. In fundamental respects got the time sequence data on the morphology, viability and induction capabilities of leucocytes, or their ability under the influence of the inductor synthesis hIFN- (or lyophilized virus hemagglutinin) higher yields of interferon, in the new proposed terms. In practical terms should consolidate that, when using a total amount of BC and from all transfusion centers in the Republic of Serbia as a raw material for the production of IFN-, in order to produce sufficient quantities IFN-, as biotherapeutic products, to cover not only needs Republic of Serbia, but to ensure the export of certain quantities of these products in countries with insufficient financial resources.
Conclusions:
1. Learn yield and enhanced the antiviral activity of hIFN- in the compositions prepared from the suspension of leukocytes obtained from the pools "buffy coat"s which are added to dissolve dextran and 10% 6% hydroxy ethyl starch high molecular weight compared to the leukocyte suspension prepared in the conventional (current) method.
2. Better and more efficient biotechnological production process hIFN-, compared to the current procedure of obtaining hIFN- according to the modified "Cantell's" method.
3. The human leukocytes used for the production of hIFN- move more and have a greater ability to induction, while the same suspension of leukocytes is used for the production of hIFN- according to the modified "Cantell's" method has a stronger capacity of cell viability and induction ability of human leukocytes.
4. The lyophilized phytohemagglutinin - PHA-P, used as an inductor for the synthesis of hIFN- , although weaker than inductor "Sendai" virus, is a good inducer of the synthesis of which has been successfully induced human leukocytes in purified suspension.
Authors Key words
humani interferon gama (hIFN-); suspenzija leukocita; "buffy coat" (BC); induktori sinze interferona
Authors Key words
human interferon gamma (hIFN-); suspension of leukocytes; "Buffy coat" (BC); interferon inducers of the synthesis
Classification
612.112:616-097(043.3)
Type
Elektronska teza
Abstract (en)
Interferons (IFN) are first described in the late fifties of the twentieth century as special proteins in the family of cytokines and induced by the host, after invasion virus. If you are transferred to the fresh tissue was observed antiviral activity, thanks to inhibit replication of the virus. In this appearance immunology known as "interference". Under normal conditions in the human body concentration of IFN-a was below detectable levels or because cells synthesize IFN or contain as its constituent element. IFN was divided in three classes: IFN-, IFN- and IFN-. Most mammalian cells formed in unequal quantities of IFN- and IFN- after stimulation with viruses or other agents, or inducers of the synthesis of IFN.
In many countries, is still detained biotechnological process for the production of human IFN (hlFN) for the clinical application of human leukocytes allocated from canned whole blood units, not older than 6 hours. After centrifugation the procedure centrifugation devotes a buffy coat (leukocyte suspension) as a "side" -produkat. Further processing of "buffy coat" (BC) was purified from the red cell contamination, in order to obtain as pure substrate of human leukocytes, which is subjected to viral infection in order to stimulate the synthesis of hIFN-. Different viruses are used as inductors synthesis hIFN-, but in practice most often used "Sendai" virus, cultivated in horion-alantoic membrane of chicken embryos. Induction of synthesis hIFN- played on temperature of +37 ° C and lasts 18 hours. Refined suspension of human tal and practical. In fundamental respects got the time sequence data on the morphology, viability and induction capabilities of leucocytes, or their ability under the influence of the inductor synthesis hIFN- (or lyophilized virus hemagglutinin) higher yields of interferon, in the new proposed terms. In practical terms should consolidate that, when using a total amount of BC and from all transfusion centers in the Republic of Serbia as a raw material for the production of IFN-, in order to produce sufficient quantities IFN-, as biotherapeutic products, to cover not only needs Republic of Serbia, but to ensure the export of certain quantities of these products in countries with insufficient financial resources.
Conclusions:
1. Learn yield and enhanced the antiviral activity of hIFN- in the compositions prepared from the suspension of leukocytes obtained from the pools "buffy coat"s which are added to dissolve dextran and 10% 6% hydroxy ethyl starch high molecular weight compared to the leukocyte suspension prepared in the conventional (current) method.
2. Better and more efficient biotechnological production process hIFN-, compared to the current procedure of obtaining hIFN- according to the modified "Cantell's" method.
3. The human leukocytes used for the production of hIFN- move more and have a greater ability to induction, while the same suspension of leukocytes is used for the production of hIFN- according to the modified "Cantell's" method has a stronger capacity of cell viability and induction ability of human leukocytes.
4. The lyophilized phytohemagglutinin - PHA-P, used as an inductor for the synthesis of hIFN- , although weaker than inductor "Sendai" virus, is a good inducer of the synthesis of which has been successfully induced human leukocytes in purified suspension.
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