Title
Svetlosnomikroskopska i imunohistohemijska studija organizacije tumorske strome kod različitih patohistoloških tipova bazocelularnog karcinoma kože čoveka
Creator
Petrović, Vladimir, 1980-
Copyright date
2015
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 21.05.2015.
Other responsibilities
mentor
Petrović, Aleksandar 1970-
član komisije
Nikolić, Ivan
član komisije
Avramović, Verica
član komisije
Mihailović, Dragan 1954-
član komisije
Anđelković, Zlatibor
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za histologiju sa embriologijom
Alternative title
Light microscopic and immunohistochemical study of tumor stroma organization in
different histopathological types of basal cell carcinoma in human integument
Publisher
[V. Petrović]
Format
147 listova
description
Histology and embryology
Abstract (en)
Previous studies of tumors mainly focused on the abnormal proliferation of tumor cells, as
the main force of this nosological entity, and on their differentiation into the new pathological
cell types. The tumor stroma, as integral component of each neoplasm, is still not sufficiently
examin ed and und ersto od . B earing in min d that b asal c ell c arcinoma o f t he s kin (BCCS) a re
stromal dependent tumors, the aim of our research was to examine the microtopographical
distribution of the antigens Ep-CAM, CD3 4 and CD9 0 in the stroma o f t he t umor r eg ions and
peritumoral skin in different histopathological types of BCCS (superficial, solid/cystic, nodular,
infiltrative, basosquamous).
The material consisted of samples of skin and subcutaneous tissue obtained after surgery,
which were routinely processed to paraffin tissue blocks, which were cut on microtome in order
to obtain thin paraffin tissue sections. The tissue sections were stained histochemically with
hematoxylin and eosin and with modified Movat’s pentachrome staining, as well as
immunohistochemically on Ep-CAM, CD34 and CD90, before being submitted to descriptive
micromophological analysis.
Cells in tumor nests of examined BCCS do not express antigens CD34 and CD90, while
the immunoreactivity toward the antigen Ep-CAM is present on the membrane of these cells in
all examined types of BCCS, with the tendency of the decrease or complete loss of this reaction
in places of squamous citodifferentiation in tumor nests. Tumor stroma of examined BCCS
shows importantly higher level of immunohistochemical reactivity toward the antigen CD90
compared to CD34 antigen. With the increase of biological aggressiveness of BCCS, it is noticed
that fibroblastoid cells of tumor stroma grow in size and number. CD34 antigen is not present in
the perinodular and internodular compartments of tumor stroma, with the exception of nodular
BCCS. Immunohistochemical reactivity toward the antigen CD90 is seen in the whole tumor
stroma, but the decrease of this immunoreactivity is noticed in infiltrative forms of BCCS
(nodular, infiltrative, basosquamous). The narrow zone of juxtatumoral region shows high degree
of immunopositivity on CD34 and CD90. The intensity of this immunohistochemical reactivity
decreases abruptly toward the periphery, to the level of intensity that dominates in the rest of skin
of the biopsy specimen. In the differentiated types of BCCS (superficial, solid/cystic) this zone of
transformation is composed of atrophic/compressed fibrous connective tissue of the reticular
dermis, while in nodular, infiltrative and basosquamous BCCS this zone is wider with the
tendency of decrease of its structure and organization.
Normal epithelial structures of skin, epidermis and epithelium of skin appendages are
CD34and CD90 immunonegative. The exceptions are the cells of outer root sheath of non vellus
hairs, which show strong CD34 immunoreactivity. Epithelial cells of secreting portions of eccrine
sweat glands show immunoreactivity toward the antigen Ep-CAM on their membranes, and Ep-
CAM is also expressed on the membranes of the cells of the outer root sheath of hair follicle, in
the parts of peritumoral skin closer to the region of tumor. CD34 immunoreactive fibroblastoid
cells are seen in periadnexal dermis around the secreting portions of eccrine sweat glands and
sebaceous glands, around the hair follicle isthmus and infundibulum, sebaceous duct, as well as
in the perimysium of arrector pili muscle. The fibroblasts of the interfascicular zone of reticular
dermis show intensive immunohistochemical reactivity toward CD34 antigen, but weak or absent
CD90 expression. The fibroblasts of the periadnexal dermis are CD90 immunoreactive around
the secreting portions of the eccrine sweat glands, sebaceous ducts and around the hair follicle
isthmus, while much weaker or even absent CD90 immunoreactivity can be seen around the
sebaceous glands, hair follicle papilla and excreting ducts of eccrine sweat glands.
Authors Key words
bazocelularni karcinom kože, tumorska stroma, peritumorska koža, Ep-CAM, CD34, CD90
Authors Key words
basal cell carcinoma of skin, tumor stroma, peritumoral skin, Ep-CAM,
CD34, CD90
Classification
616.5-006.6-076.5-097
Type
Tekst
Abstract (en)
Previous studies of tumors mainly focused on the abnormal proliferation of tumor cells, as
the main force of this nosological entity, and on their differentiation into the new pathological
cell types. The tumor stroma, as integral component of each neoplasm, is still not sufficiently
examin ed and und ersto od . B earing in min d that b asal c ell c arcinoma o f t he s kin (BCCS) a re
stromal dependent tumors, the aim of our research was to examine the microtopographical
distribution of the antigens Ep-CAM, CD3 4 and CD9 0 in the stroma o f t he t umor r eg ions and
peritumoral skin in different histopathological types of BCCS (superficial, solid/cystic, nodular,
infiltrative, basosquamous).
The material consisted of samples of skin and subcutaneous tissue obtained after surgery,
which were routinely processed to paraffin tissue blocks, which were cut on microtome in order
to obtain thin paraffin tissue sections. The tissue sections were stained histochemically with
hematoxylin and eosin and with modified Movat’s pentachrome staining, as well as
immunohistochemically on Ep-CAM, CD34 and CD90, before being submitted to descriptive
micromophological analysis.
Cells in tumor nests of examined BCCS do not express antigens CD34 and CD90, while
the immunoreactivity toward the antigen Ep-CAM is present on the membrane of these cells in
all examined types of BCCS, with the tendency of the decrease or complete loss of this reaction
in places of squamous citodifferentiation in tumor nests. Tumor stroma of examined BCCS
shows importantly higher level of immunohistochemical reactivity toward the antigen CD90
compared to CD34 antigen. With the increase of biological aggressiveness of BCCS, it is noticed
that fibroblastoid cells of tumor stroma grow in size and number. CD34 antigen is not present in
the perinodular and internodular compartments of tumor stroma, with the exception of nodular
BCCS. Immunohistochemical reactivity toward the antigen CD90 is seen in the whole tumor
stroma, but the decrease of this immunoreactivity is noticed in infiltrative forms of BCCS
(nodular, infiltrative, basosquamous). The narrow zone of juxtatumoral region shows high degree
of immunopositivity on CD34 and CD90. The intensity of this immunohistochemical reactivity
decreases abruptly toward the periphery, to the level of intensity that dominates in the rest of skin
of the biopsy specimen. In the differentiated types of BCCS (superficial, solid/cystic) this zone of
transformation is composed of atrophic/compressed fibrous connective tissue of the reticular
dermis, while in nodular, infiltrative and basosquamous BCCS this zone is wider with the
tendency of decrease of its structure and organization.
Normal epithelial structures of skin, epidermis and epithelium of skin appendages are
CD34and CD90 immunonegative. The exceptions are the cells of outer root sheath of non vellus
hairs, which show strong CD34 immunoreactivity. Epithelial cells of secreting portions of eccrine
sweat glands show immunoreactivity toward the antigen Ep-CAM on their membranes, and Ep-
CAM is also expressed on the membranes of the cells of the outer root sheath of hair follicle, in
the parts of peritumoral skin closer to the region of tumor. CD34 immunoreactive fibroblastoid
cells are seen in periadnexal dermis around the secreting portions of eccrine sweat glands and
sebaceous glands, around the hair follicle isthmus and infundibulum, sebaceous duct, as well as
in the perimysium of arrector pili muscle. The fibroblasts of the interfascicular zone of reticular
dermis show intensive immunohistochemical reactivity toward CD34 antigen, but weak or absent
CD90 expression. The fibroblasts of the periadnexal dermis are CD90 immunoreactive around
the secreting portions of the eccrine sweat glands, sebaceous ducts and around the hair follicle
isthmus, while much weaker or even absent CD90 immunoreactivity can be seen around the
sebaceous glands, hair follicle papilla and excreting ducts of eccrine sweat glands.
“Data exchange” service offers individual users metadata transfer in several different formats. Citation formats are offered for transfers in texts as for the transfer into internet pages. Citation formats include permanent links that guarantee access to cited sources. For use are commonly structured metadata schemes : Dublin Core xml and ETUB-MS xml, local adaptation of international ETD-MS scheme intended for use in academic documents.