Title
Morfološka, morfometrijska i imunohistohemijska analiza promena vezivnotkivnih omotača humanog suralnog nerva u toku starenja
Creator
Kundalić, Braca K.
Copyright date
2015
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 03.06.2015.
Other responsibilities
mentor
Ugrenović, Slađana
član komisije
Čukuranović, Rade
član komisije
Rančić, Gorana
član komisije
Jovanović, Ivan
član komisije
Srdić-Galić, Biljana 1972-
Academic Expertise
Medicinske nauke
University
Univerzitet u Nišu
Faculty
Medicinski fakultet
Group
Katedra za anatomiju
Alternative title
Morphological, morphometric and immunohistochemical analysis of the changes in the connective tissue sheaths of human sural nerve during ageing
Publisher
[B. К. Kundalić]
Format
137 listova
description
Anatomy
Abstract (en)
Structural and functional age-related changes of peripheral nerve influence the regenerative capacity of
the nerve. Stroma of the peripheral nerve acts as a support that maintains optimal microenvironment for the
nerve impulse conduction, as well as the scaffold for the nerve fibers regeneration.
Tissue samples of human sural nerve were analyzed using morphological, morphometric and
immunohistochemical methods. The nerves were harvested during the autopsies and after lower limb
amputation of the patients with diabetes mellitus and peripheral vascular disease. This study investigated the
structural changes of the connective tissue sheaths during the ageing, as well as presence, localization and
quantification of collagen IV, collagen I and laminin.
Results of this study showed the presence of marked age-related changes in the perineurium and
endoneurium of the investigated nerves, unlike epineurial sheath and fascicular structure of the nerve. Ageing is
related to significant remodelling of the extracellular matrix of the perineurial sheath. Increased amount of
collagen IV, as well as decreased expression of collagen I and laminin, were observed. Axonal atrophy in
endoneurium were followed by more intense fibrosis and significant increase of collagen IV, as well as
decreased amount of collagen I and laminin during ageing. In diabetic group we found significant endoneurial
fibrosis with higher levels of collagen IV compared to the control, while patients with peripheral vascular
disease suffered more prominent changes in the perineurial layer than age-matched control group, followed by
increased deposition of collagen IV, collagen I and laminin.
Observed differences in changes of the endoneurial environment and the perineurial sheath between
senile, diabetic and vascular peripheral neuropathy further suggest the importance of extracellular matrix of
nerve, and may contribute to better understanding of regeneration and functional recovery of nerve after injury,
offering the guidelines to the future studies in the field of neurosciences and bioengineering.
Authors Key words
suralni nerv, starenje, kolagen, laminin
Authors Key words
Sural nerve, ageing, collagen, laminin
Classification
611.835:612.67(043.3)
Type
Tekst
Abstract (en)
Structural and functional age-related changes of peripheral nerve influence the regenerative capacity of
the nerve. Stroma of the peripheral nerve acts as a support that maintains optimal microenvironment for the
nerve impulse conduction, as well as the scaffold for the nerve fibers regeneration.
Tissue samples of human sural nerve were analyzed using morphological, morphometric and
immunohistochemical methods. The nerves were harvested during the autopsies and after lower limb
amputation of the patients with diabetes mellitus and peripheral vascular disease. This study investigated the
structural changes of the connective tissue sheaths during the ageing, as well as presence, localization and
quantification of collagen IV, collagen I and laminin.
Results of this study showed the presence of marked age-related changes in the perineurium and
endoneurium of the investigated nerves, unlike epineurial sheath and fascicular structure of the nerve. Ageing is
related to significant remodelling of the extracellular matrix of the perineurial sheath. Increased amount of
collagen IV, as well as decreased expression of collagen I and laminin, were observed. Axonal atrophy in
endoneurium were followed by more intense fibrosis and significant increase of collagen IV, as well as
decreased amount of collagen I and laminin during ageing. In diabetic group we found significant endoneurial
fibrosis with higher levels of collagen IV compared to the control, while patients with peripheral vascular
disease suffered more prominent changes in the perineurial layer than age-matched control group, followed by
increased deposition of collagen IV, collagen I and laminin.
Observed differences in changes of the endoneurial environment and the perineurial sheath between
senile, diabetic and vascular peripheral neuropathy further suggest the importance of extracellular matrix of
nerve, and may contribute to better understanding of regeneration and functional recovery of nerve after injury,
offering the guidelines to the future studies in the field of neurosciences and bioengineering.
“Data exchange” service offers individual users metadata transfer in several different formats. Citation formats are offered for transfers in texts as for the transfer into internet pages. Citation formats include permanent links that guarantee access to cited sources. For use are commonly structured metadata schemes : Dublin Core xml and ETUB-MS xml, local adaptation of international ETD-MS scheme intended for use in academic documents.
